Table 1.
Investigated proteins and lipids
| Protein | Structure | RLit (nm) | D (± SD) μm2 s−1 (direct addition) | D (± SD) (μm2 s−1) (SNARE-mediated fusion) |
|---|---|---|---|---|
| DPPE (lipid) | monomer | 0.4 | 11.58 (± 0.57) | — |
| Cytochrome B5 | monomer | 0.7 | 10.24 (± 0.63) | 10.43 (± 0.76) |
| KcsA | monomer | 1.2 | 9.27 (± 0.53) | — |
| tetramer | 2.4 | — | 7.96 (± 0.53) | |
| EcClC | monomer | 1.8 | 8.49 (± 0.46) | — |
| dimer | 2.8 | — | 7.51 (± 0.84) | |
| AcrB | monomer | 2.1 | 8.47 (± 0.75) | — |
| trimer | 3.6 | — | 7.23 (± 0.39) |
The estimated radii, RLit, were taken from the respective crystal structures. Diffusion coefficients, D, were determined by 2fFCS. All diffusion measurements were performed at 22°C. SD, standard deviation.