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. 2013 Jul 16;105(2):481–493. doi: 10.1016/j.bpj.2013.05.046

Figure 6.

Figure 6

Effects of temperature on structural motifs in WIPC. (A) CD curves for a 6.7 μM WIPC sample at 278 (black), 293 (blue), and 308 (red) K. An increase (decrease) in signal is observed for the 215–230 (195–205) range. (B and C) Temperature effects on the intrinsic 13C′ shifts of WIPC as observed in CON-IPAP spectra. Black (red) peaks were obtained at 278 (308) K; dark (light) peaks were obtained for samples under normal (denaturing) conditions. Plots focus on residues A425 (B) and T471 (C). (D–F) 13C′ IRCSs at 308 K (D) and 278 K (E), and temperature factors (F) plotted against the WIPC sequence. The intrinsic temperature coefficient was calculated from the slope of IRCS versus temperature in the 278–308 range. The first residues of Pro-Pro dyads are designated by black triangles. Typical errors for temperature factors were 0.2 and 0.5 ppb/K for the unstructured and structured regions of WIPC, respectively.