The peptide AngioT (DRVYIHPFHL) was labeled with TMT-127a, -127b, -129a, and -129b. Following labeling, the differentially tagged peptides were mixed at 10:1 ratios and analyzed with a FTMS2 HCD scan on an Orbitrap Elite. Spectra were collected at a range of resolving powers (15kâ240k @ 400 m/z). With the D20 Orbitrap analyzer, a relatively short transient of 96ms provides baseline resolution between the differentially expressed reporter ions.