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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: Anal Chem. 2012 Aug 20;84(17):7469–7478. doi: 10.1021/ac301572t

Figure 2.

Figure 2

The peptide AngioT (DRVYIHPFHL) was labeled with TMT-127a, -127b, -129a, and -129b. Following labeling, the differentially tagged peptides were mixed at 10:1 ratios and analyzed with a FTMS2 HCD scan on an Orbitrap Elite. Spectra were collected at a range of resolving powers (15k–240k @ 400 m/z). With the D20 Orbitrap analyzer, a relatively short transient of 96ms provides baseline resolution between the differentially expressed reporter ions.