Skip to main content
. Author manuscript; available in PMC: 2013 Jul 18.
Published in final edited form as: Pathol Res Pract. 2011 Sep 8;207(9):545–553. doi: 10.1016/j.prp.2011.06.001

Fig. 1.

Fig. 1

siRNA-AAH inhibits AAH expression and directional motility in HTR-8/SVneo cells. HTR-8/SVneo cells were transiently transfected with siRNA-Scr or siRNA-AAH, and AAH (A) mRNA and (B) protein were measured by qRT-PCR and ELISA, and (C–F) directional motility was measured using the ATP luminescence-based motility-invasion assay. AAH mRNA levels were normalized to 18S rRNA, and immunoreactivity was normalized to protein content (NanoOrange assay). For the directional motility assays, we calculated percentages of (C) motile (adherent + non-adherent), (D) sessile, (E) motile adherent, and (F) motile non-adherent cells in eight replicate assay chambers. Graphs depict the mean ± S.E.M. levels. Inter-group comparisons were made using Student t-tests.