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. Author manuscript; available in PMC: 2013 Jul 18.
Published in final edited form as: Pathol Res Pract. 2011 Sep 8;207(9):545–553. doi: 10.1016/j.prp.2011.06.001

Fig. 5.

Fig. 5

In vivo siRNA-AAH inhibition of Notch-Signaling Network – ELISA Studies. The implantation sites (mesometrial triangles) of GD17 pregnant Long Evans rats were microinjected with siRNA-AAH or siRNA-Scr, and 24 hours later, placental and mesometrial tissues were micro-dissected and homogenized in RIPA buffer. ELISA was used to measure levels of (A) AAH, (B) Notch-1, (C) Jagged-1, and (D) p85 (control) immunoreactivity. Results were normalized to protein content in each well. Graphs depict the mean ± S.E.M. corresponding to the levels of immunoreactivity in eight samples per group (all assays were performed in triplicate). Inter-group comparisons were made using two-way ANOVA with Bonferroni posttests. Significant P-values are shown within each panel.