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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2013 May 24;24(7):1045–1052. doi: 10.1007/s13361-013-0637-6

Figure 3.

Figure 3

(a) CID of the ion/ion complex formed using the trimer reagent cluster, [M+3H+3R3Na-Na]2+, results in up to 3 sulfo-NHS signature neutral losses. (b) Subsequent CID of the second sulfo-NHS loss confirms only a third signature loss and concomitant analyte derivatization is possible with the trimer reagent. (c) CID of the ion/ion complex formed using the tetramer reagent cluster, [M+3H+4R4Na-Na]2+, results in up to 4 sulfo-NHS signature neutral losses. (d) Subsequent CID of the second sulfo-NHS loss confirms that only up to four modifications are possible with the tetramer reagent cluster.