Fig. 2.
Effects of caffeine on mRNA expression of CYP1A2. Primary human hepatocytes (A) or rat hepatocytes (B) were treated with vehicle (DMSO), caffeine (50–400 μM for human hepatocytes; 50 μM for rat hepatocytes), omeprazole (100 μM), or 3-MC (0.5 μM) for 72 hr. mRNA expression levels of CYP1A2 were determined by qRT-PCR. The data shown are representative of results obtained from 4 and 2 different batches of human and rat hepatocytes, respectively (mean ± S.D.; n = 3 wells/drug treatment). *, p < 0.05 ; ***, p < 0.001 compared with vehicle-treated group.