Table 5S.
Operational conditions of TD-GC-TOF MS system for the analysis of fresh and decaying strawberry.
| [A] Sampling information of strawberry volatiles | |||
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| a. Information of strawberry for sampling | |||
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| Order | Sample code | Storage time (day) | Storage temp. (°C ) |
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| 1 | SRD-0 | 0 | 25 |
| 2 | SRD-1 | 1 | 25 |
| 3 | SRD-3 | 3 | 25 |
| 4 | SRD-6 | 6 | 25 |
| 5 | SRD-9 | 9 | 25 |
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| Initial weight = 50.09 g | |||
| b. Sampling approach | |
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| Sampler: | 3 bed sorbent tube |
| Purge gas: | Nitrogen (>99.999%) |
| Purge gas flow: | 50 mL·min−1 |
| Pump model: | MP-Σ30 (Sibata, Japan) |
| Heater model: | TC200P (Korea) |
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| [B] Instrumental setups for VOC analysis | |||
|---|---|---|---|
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| a. GC (Shimadzu GC-2010, Japan) and MS (Shimadzu GCMS-QP2010, Japan) Column: CP Wax (diameter: 0.25 mm, length: 60 m, and film thickness: 0.25 μm ) | |||
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| Oven setting | Detector setting | ||
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| Initial temp: | 35 °C (10 min) | Ionization mode: | EI (70 eV) |
| Ramp rate: | 6 °C·min −1 | Ion source temp.: | 200 °C |
| Max oven temp: | 215 °C (10 min) | Interface temp.: | 200 °C |
| Total time: | 50 min | TIC scan range: | 35∼260 m/z |
| Carrier gas: | He (99.999%) | ||
| Carrier gas flow: | 1 mL·min −1 | ||
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| b. Thermal desorber (Unity, Markes, UK) | |||
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| Cold trap sorbent: | Tenax TA + Carbopack B (volume ratio=1:1) (diameter: 2 mm and sorbent bed length = 5 mm) | ||
| Split ratio: | 1:5 | Adsorption temp.: | -10 °C |
| Split flow: | 5 mL·min−1 | Desorption temp.: | 320 °C |
| Trap hold time: | 20 min | Flow path temp: | 150 °C |
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| c. Sorbent (Sampling) Tube | |||
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| Sorbent material: | Tenax TA + Carbopack B + Carboxen 1000 (mass (mg)=100 : 100: 100 ) | ||
| Desorption flow: | 50 mL·min−1 | ||
| Desorption time: | 5 min | Desorption temp.: | 300 °C |