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. 2013 May 27;13(6):6981–7003. doi: 10.3390/s130606981

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 5. — Iron-Based Magnetic Nanoparticles (MNP). (A) The paramagnetic core of the modified MNP consists of an iron oxide particle 10 nm in diameter and possessing a Fe₂O₃:FeO ratio of 2:1. This iron oxide core is enclosed within a dextran coat, thus providing numerous sites for surface modification. This allows conjugation of the MNP to an appropriate ligand of a specific biomolecular target. In this specific case, the MNP is conjugated to a unique dual peptide construct consisting of a 15-residue domain with high affinity for P-selectin linked to a second 24-residue thrombin-binding domain. The P-selectin binding domain consists of the primary amino acid sequence LVSVLDLEPLDAAWL and was discovered through the use of phage display technology. (B) Hematoxylin and eosin coronal section (7 micron slice thickness, bregma +1.00 mm) 26 hours after ischemia/reperfusion and two hours after MNP-PBPl injection. (i) Ipsilateral cortex, Cy5.5-anti-P-selectin-IgG, (ii) Ipsilateral striatum, (iii) Contralateral cortex, (iv) Contralateral striatum, (v) T2 map coronal slice (bregma +1.18 mm) showing the infarct as a light blue area with yellow-green center [87].

Figure 5. — Iron-Based Magnetic Nanoparticles (MNP). (A) The paramagnetic core of the modified MNP consists of an iron oxide particle 10 nm in diameter and possessing a Fe₂O₃:FeO ratio of 2:1. This iron oxide core is enclosed within a dextran coat, thus providing numerous sites for surface modification. This allows conjugation of the MNP to an appropriate ligand of a specific biomolecular target. In this specific case, the MNP is conjugated to a unique dual peptide construct consisting of a 15-residue domain with high affinity for P-selectin linked to a second 24-residue thrombin-binding domain. The P-selectin binding domain consists of the primary amino acid sequence LVSVLDLEPLDAAWL and was discovered through the use of phage display technology. (B) Hematoxylin and eosin coronal section (7 micron slice thickness, bregma +1.00 mm) 26 hours after ischemia/reperfusion and two hours after MNP-PBPl injection. (i) Ipsilateral cortex, Cy5.5-anti-P-selectin-IgG, (ii) Ipsilateral striatum, (iii) Contralateral cortex, (iv) Contralateral striatum, (v) T2 map coronal slice (bregma +1.18 mm) showing the infarct as a light blue area with yellow-green center [87].