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. Author manuscript; available in PMC: 2014 Jun 20.
Published in final edited form as: Chem Biol. 2013 Jun 20;20(6):753–763. doi: 10.1016/j.chembiol.2013.05.008

Figure 3.

Figure 3

Kalihinol F phenotype is prevented by addition of copper. a. 5 hpf embryos were treated with either DMSO or 2.5 μg/ml kal F then rescued at 7 hpf with 10 μM CuCl2, Zn(OAc)2 and embryo water (e H2O). Only copper-treated embryos showed normal pigmentation, notochord and hindbrain comparable to control embryos (bottom row, second and fourth figures from left). b. Normal hematopoiesis and rescue of notochord, somites, spinal cord with copper were confirmed by o-dianisidine staining and in situ for ntl, myod, hnf6. See also Figure S1.