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. 2013 Jul 18;9(7):e1003601. doi: 10.1371/journal.pgen.1003601

Figure 7. CTCF overexpression in WT, UFM and FXS fibroblasts.

Figure 7

CTCF overexpression in WT, UFM and FXS cell lines after transfection with a commercial vector containing the open reading frame of variant 1 of CTCF. Quantitative RT-PCR showed a strong increase of CTCF mRNA after 48 and 120 hours from transfection (A), while levels of FMR1 transcription remained substantially unchanged (B). The levels of CTCF transcription in the untreated cells were arbitrarily set at 1 as well as those of FMR1 transcript in WT and UFM fibroblasts, while those of FMR1-mRNA in FXS cells were set at 0. Bars indicate standard deviation.