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. 2013 Jul 18;9(7):e1003601. doi: 10.1371/journal.pgen.1003601

Figure 9. Histone H3 methylation analysis after CTCF knock-down.

Figure 9

ChIP analysis of H3-K4 (A) and H3-K9 (B) methylation in WT fibroblasts after CTCF knock-down with FMR1 reduction. Each box-plot depicts the amount (ng) of IP-DNA in promoter and exon 1 regions in control (UT) and siRNA transfected (siRNA) cells. The levels of H3-K4 methylation were significantly reduced in both regions (p<0.05), while those of H3-K9 were increased particularly in the promoter. Box-plots indicate the mean of at least three independent experiments, the corresponding standard error and standard deviation (thin lines). ChIP experiments included negative controls performed by IgG immunoprecipitation and no template control (not shown).