Figure 4. SOLO co-aligns with C(3)G and SMC1 on chromosome arms in pro-oocytes and oocytes.

Expression of Venus::SOLO was induced by nos-GAL4::VP16 in Df(2L)A267/solo^Z2-0198; {UASp-Venus::SOLO}/{nos-GAL4::VP16} females and stained with anti-GFP antibody. SC was visualized by anti-C(3)G staining and DNA was stained with DAPI. Scale bars: 5 µm. (A) SOLO and C(3)G staining in a whole-mount germarium. Venus::SOLO localized to pro-nurse cells (no C(3)G staining, arrowheads) and to pro-oocytes. Faint linear Venus::SOLO signals could be discerned in some pro-oocytes and oocytes (arrows). (B–C) Venus::SOLO localization in germ cell nuclei prepared by chromosome spread method. Linear Venus::SOLO signals aligned with linear C(3)G staining (arrow) in pro-oocytes. Venus::SOLO also localized as bright foci and thin lines in pro-nurse cells (arrowheads). (C) Magnification of a pro-oocyte marked by the arrow in (B). (D and E) Co-alignment of SOLO and SMC1 signals in germ cell nuclei. Chromosomes were prepared by chromosome spreading. Pro-oocyte (arrow) and pro-nurse cell (arrowhead) are marked. (E) Magnification of a pro-oocyte marked by the arrow in (D). Arrowheads point to SC segments with well-aligned SOLO and SMC1 signals in which SMC1 signal is significantly brighter than SOLO signal.