Figure 5. solo mutations destabilize lateral elements and central regions of SCs.

Scale bar: 5 µm. (A–D) solo mutations caused defective C(3)G staining. Pro-oocytes and oocytes were stained by anti-ORB antibody. SC was visualized by anti-C(3)G staining and DNA was stained with DAPI. (A) In WT germaria C(3)G formed linear structures in nuclei of ORB-enriched cells within region 2a, 2b and 3, and was restricted to the oocyte in region 3. (B, C) In solo (solo^Z2-3534/Df(2L)A267) germaria, pro-oocytes and oocytes were marked by enriched ORB staining. C(3)G staining patterns included spotted and fragmented (B) and normal-like (C) and are displayed in magnifications of cells marked with arrowheads. Note also the absence of C(3)G staining in the cyst in region 3. (Note: nuclei that appeared fully stained with C(3)G and did not exhibit obvious fragmentation were classified as “normal-like” even if the staining pattern did not look completely normal.) (D) Quantification of C(3)G defects in solo and solo ord pro-oocytes and oocytes. The graph shows the percentages of nuclei from ORB-enriched cells with normal-like, fragmented, spotted and no C(3)G staining. The numbers of pro-oocytes or oocytes scored are noted for each bar. (E) Abnormal SMC1 and C(3)G linear structures in solo pro-oocytes prepared by chromosome spreading and stained with both anti-SMC1 and anti-C(3)G antibodies. SMC1 exhibited normal-like, fragmented and spotted staining patterns that closely paralleled the patterns of C(3)G staining.