Table 2. Sister chromatid versus homolog NDJ for chromosome 2 in solo females.
Progeny Phenotypes | Egg genotypes | NDJ type | soloZ2-0198 | soloZ2-3534 |
+ | b/cn bw | Homolog | 1012 | 259 |
Bw | b bw/cn bw | Homolog | 36 | 10 |
b | b/b | Sister | 144 | 38 |
cn bw | cn bw/cn bw | Sister | 106 | 7 |
Cn | cn/cn bw | Sister | 37 | 4 |
bw sp | nullo-2 | Both | 360 | 256 |
solo, cn bw/b vas7 females were crossed singly to two C(2)EN, bw sp males. 2.48 and 3.02 progeny per female were recovered in the Z2-0198 and Z2-3534 crosses, respectively, indicating elevated NDJ frequencies. The phenotypes of the progeny, their presumed genotypes and origins and the numbers recovered are shown in the table. See Figure S2 for classifications of NDJ types. %S NDJ = 100×Sister/(Sister+Homolog). Correcting for viability effects, %S = 100× (144/0.5176+106/0.6349+37)/((144/0.5176+106/0.6349+37)+(1012+36)) = 32%. See Materials and Methods for methodology for viability correction. The viability of soloZ2-3534 cn bw homozygotes was not measured but they appeared to be poorly viable because they were rarely found in the soloZ2-3534 cn bw/CyO stock. Therefore the estimate of %S NDJ was based solely on the soloZ2-0198 cross.