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. 2013 Jul 18;9(7):e1003591. doi: 10.1371/journal.pgen.1003591

Figure 1. Dissection of genetic interactions between gen-1, mus-81, slx-1, xpf-1, him-6 and slx-4.

Figure 1

(A) gen-1 does not alter viability, alone or in conjunction with nucleases, slx-4 or him-6. Progeny viability in % was determined as described in Material and Methods. (B) Evidence for MUS-81/SLX-1 and XPF-1/HIM-6 acting in two genetic pathways. Scoring was done as in A. For double mutants with reduced viability the F1 progeny of double homozygous mothers derived from hT2-balanced lines was scored (for strain list see Materials and Methods). In contrast slx-1; him-6 double mutants derived from a strain were him-6 is balanced by nT1 behave as synthetic lethal (data not shown) [47]. We think that this lethality is due the nT1 balancer, which has been described as prone to brake down, as preferably segregating to the male germ line and as conferring a reduced brood size [88]. (C) Reduced progeny viability of slx-4 is not enhanced by nuclease mutations. (D) Genetic model. Asterisks indicate statistical significance between different genotypes as determined by two-tailed Mann-Whitney test (*** indicates P<0.01).