Figure 5. Chromosome linkages depend on SPO-11-induced meiotic double-strand breaks.

(A) Images of DAPI-stained -1 and -2 oocytes (position distal to spermatheca) of the indicated genotypes. Scale bars are 4 µm unless indicated otherwise. (B) Irradiation induced double-strand breaks bypass the SPO-11 requirement. Red arrows indicate chromosome linkages. Blue arrows indicate DNA fragments, which we observed in [slx-1; him-6 spo-11] but not in [slx-1; him-6]. Scale bars are shown in white (5 µm).