Figure 6. Yki is required for scrib ⁻+ab tumour overgrowth.

ey-FLP induced eye/antennal disc clones marked by GFP (white, or green in merges). Elav, Dac and Eya are in white (blue in merged images, changing to dark blue when overlaid with GFP), and F-actin for cell morphology is in red. Brain lobes are labeled BL. GFP (panels A–C,G,H), Elav (panels A′–C′), Dac (panel G′), Eya (panel H′) and merges (panels A″–C″,D,E,G″,H″). (A) yki^RNAi-expressing clones. (B) Clones overexpressing yki^RNAi+ab are similar to ab overexpressing clones. (C–E) Expressing yki^RNAi in scrib¹+ab tumours does not restore Elav expression to the tumour cells (C, arrowhead), however, tumour overgrowth is substantially reduced (E, compared to scrib¹+ab tumours in D), and larvae pupate instead of entering an extended larval stage. (F) Quantification of percentage of scrib¹+ab and scrib¹+ab+yki^RNAi tumour-bearing larvae that had pupated by 9 days AEL. (G,H) Expressing yki^RNAi in scrib¹+ab tumours restores Dac expression in the clones (G, arrowhead) but Eya levels remain reduced (H, arrowhead). Yellow scale bar = 50 µm.