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. 2013 Jul 18;9(7):e1003623. doi: 10.1371/journal.pgen.1003623

Figure 6. Induction of the SOS response.

Figure 6

The ß-galactosidase specific activities from the SOS transcriptional reporter fusion dinC-lacZ in strains with ICEBs1 in the indicated secondary attachment sites are presented. Strains were grown as described in Figure 4 and samples for ß-galactosidase assays were taken 3 hours after induction of ICEBs1 gene expression. Data presented are the averages of two biological replicates (four for ΔattR strain KM392)). For all of the strains with insertions in secondary attachment sites, the values from the biological replicates were within 20% of the average. Strains used include: wt, attB::ICEBs1 (KM390); ykrP::ICEBs1 (KM402); mmsA::ICEBs1 (KM394); attB::ICEBs1 ΔattR::tet (KM392); srfAA::ICEBs1 (KM400); yvbT::ICEBs1 (KM396); yrkM::ICEBs1 (KM404).