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. 2013 Jul 18;8(7):e69196. doi: 10.1371/journal.pone.0069196

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© 2013 In et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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EAEC-L stimulates Stx1 uptake in T84 cells by stimulation of MPC.

(A) Representative IB and quantitative representations of IB data show that increasing concentrations of EAEC-L significantly increased Stx1 uptake in T84 cells compared to untreated cells (n ≥ 3 monolayers per each experimental condition; * - significant compared to the control (p < 0.05)). (B) Representative XY optical sections through either control or EAEC-L-treated T84 cells additionally incubated with Stx1B-488 for 4 h show EHEC-L induced actin remodeling with formation of F-actin coated macropinosomes (spherical or irregularly shaped). Numerous macropinosomes carry the Stx1B-488 (green). F-actin - red by phalloidin -Alexa Fluor 568; bars -5 µm.