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. 2013 Jul 18;8(7):e69180. doi: 10.1371/journal.pone.0069180

Table 1. List of the SNPs analyzed along with PCR primers and techniques used for genotyping.

SNP (rs ID) Amino acidChange Techniqueadopted Forward Primer/Reverse Primer (5′-3′) Annealingtemperature FragmentSize (bp)
203 G>A (rs2066472) R68Q PCR-RFLP(Taq I) CCCTGCTTGGAGGGCAGTGC GCAGATCAGATGACCCACTCTGCCT 67°C 351
1298 A>C (rs1801131) E429A PCR-RFLP(Mbo II) CTGCCCTCTGTCAGGAGTGTGC3’/CCCTTCTCCCTTTGCCATGTCCA3’ 65°C 368
1793 G>A (CM056008NOIATAUM_DMCH) R594Q Sequencing GTGATACTGGCAGTGGGCCTTGT CTCTCGCATTCTGGGTGGGC 61°C 332
Intronic (rs3818762) Sequencing GTGATACTGGCAGTGGGCCTTGT CTCTCGCATTCTGGGTGGGC 61°C 332