Table 1. List of the SNPs analyzed along with PCR primers and techniques used for genotyping.
SNP (rs ID) | Amino acidChange | Techniqueadopted | Forward Primer/Reverse Primer (5′-3′) | Annealingtemperature | FragmentSize (bp) |
203 G>A (rs2066472) | R68Q | PCR-RFLP(Taq I) | CCCTGCTTGGAGGGCAGTGC GCAGATCAGATGACCCACTCTGCCT | 67°C | 351 |
1298 A>C (rs1801131) | E429A | PCR-RFLP(Mbo II) | CTGCCCTCTGTCAGGAGTGTGC3’/CCCTTCTCCCTTTGCCATGTCCA3’ | 65°C | 368 |
1793 G>A (CM056008NOIATAUM_DMCH) | R594Q | Sequencing | GTGATACTGGCAGTGGGCCTTGT CTCTCGCATTCTGGGTGGGC | 61°C | 332 |
Intronic (rs3818762) | – | Sequencing | GTGATACTGGCAGTGGGCCTTGT CTCTCGCATTCTGGGTGGGC | 61°C | 332 |