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. 1980 Nov;66(5):1101–1108. doi: 10.1172/JCI109939

Phorbol diester-induced macrophage differentiation of leukemic blasts from patients with human myelogenous leukemia.

H P Koeffler, M Bar-Eli, M Territo
PMCID: PMC371548  PMID: 6253522

Abstract

Phorbol esters, including 12-O-tetradecanoylphorbol 13-acetate (TPA), induce terminal macrophagelike differentiation of cells from human acute myelogenous leukemia lines. We report that myelogenous leukemia cells obtained from patients undergo macrophagelike differentiation after exposure to TPA. The myeloid leukemic cell cultured with TPA became adherent to charged surfaces with long filamentous pseudopodia; developed positive staining for alpha-napthyl acetate esterase, increased lysozyme secretion, reduced nitroblue tetrazolium, and acquired the ability to phagocytose candida. Cells from patients with lymphocytic leukemia did not become macrophagelike when cultured with TPA.

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Selected References

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