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. 2013 Jul 18;8(7):e68614. doi: 10.1371/journal.pone.0068614

Figure 5. Inhibition of GSK-3β activity enhanced migration of NPC cell lines.

Figure 5

(A) Representative images showing the cell covered area on the culture plate containing NPC cells after transfection with GSK3β plasmid. Inhibition of GSK-3β by GSK3β-KD transfection enhanced migration of CNE-1 and CNE-2 cells, whereas activation of GSK-3β by GSK3β-CA transfection suppressed migration of CNE-1 and CNE-2 cells; (B) Quantitative analyses for the cell covered areas showed that the migrated cells in the GSK3β-KD group increased significantly, whereas those in GSK3β-CA group decreased significantly when compared to the control. Migration of NPC cells was evaluated by scratch assay after GSK3β-KD or CA plasmid (2 μg/mL) transfection for 24 or 48 h. The data indicate the means (SEM) of 3 independent experiments. NC: normal control; CA: constitutively active GSK3β plasmid; KD: kinase-dead GSK3β plasmid.