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. 2013 Jul 18;8(7):e68614. doi: 10.1371/journal.pone.0068614

Figure 7. GSK3β-enhanced migration and invasion of NPC cells were abrogated by EZH2 siRNA transfection.

Figure 7

(A–C) The siRNA knockdown efficiency was evaluated by testing the protein level of EZH2. EZH2 siRNA transfection significantly reduced EZH2 expression in CNE-1 and CNE-2 cells; (D,E) Inhibition of EZH2 by EZH2 siRNA (50 nmol/L) transfection significantly inhibited migration of CNE-1 and CNE-2; (F,G) EZH2 siRNA (50 nmol/L) transfection significantly inhibited GSK3β-KD-dependent migration of CNE-1 and CNE-2; (H,I) Inhibition of EZH2 by EZH2 siRNA (50 nmol/L) transfection significantly inhibited invasion of CNE-1 and CNE-2; (J,K) EZH2 siRNA transfection significantly inhibited GSK3β-KD-dependent invasion of CNE-1 and CNE-2. Migration and invasion of NPC cells were evaluated after EZH2 siRNA (50 nmol/L) and/or GSK3β-KD plasmid (2 μg/mL) were transfected for 48 or 72 h. The data indicate the means (SEM) of 3 independent experiments. NC: normal control; KD: kinase-dead GSK-3β plasmid.