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. 2013 Jul 18;8(7):e66672. doi: 10.1371/journal.pone.0066672

Figure 3. Altered expression of UPR-target genes in mutant SOD1 transgenic mice upon deletion of ATF4.

Figure 3

(A) Expression levels of several ER stress-responsive proteins were monitored in spinal cord extracts derived from ATF4+/+, ATF4−/−, ATF4+/+/SOD1G86R and ATF4−/−/SOD1G86R symptomatic mice using Western blot analysis. The image was composed from representative bands cropped from the same gel and film. (B) Quantification of experiments presented in A. ATF4+/+ (N = 3), ATF4−/− (N = 3), ATF4+/+/SOD1G86R (N = 6) and ATF4−/−/SOD1G86R (N = 3). HSP90 was used as loading control, and values were normalized to the average signal obtained in ATF4+/+ mice. (C) In parallel, CHOP and BIM protein levels were monitored in animals described in A. Mean and standard error is presented. *: p<0.05, calculated with Student's t-test. n.s.: non significant.