Table 4. Sequence comparisons of bands excised from DGGE gels (Fig. 1 C and D) and blasted against the 16S rRNA gene database retrieved from SILVA.
Mycobacterium genus specific primers (JSY16S) DGGE | Slow growing Mycobacteria specific primers (APTK16Slow) DGGE | ||||||||
DGGE band | Identity (%) | E value | Match accession number | Match name | DGGE band | Identity (%) | E value | Match accession number | Match name |
1A | 98.08 | 0 | DQ249999 | Mycobacterium sp. L47 | 2A | 99.42 | 0 | GQ153275 | Mycobacterium colombiense |
1B | 99.73 | 0 | EU165538 | Mycobacterium brasiliensis | 2B | 97.28 | 3×10−168 | GQ153275 | Mycobacterium colombiense |
1C | 99.44 | 0 | X93029 | Mycobacterium sp. | 2C | 99.71 | 0 | FJ794352 | Mycobacterium sp. NLA000202017 |
1D | 100 | 2×10−64 | EF019937 | Uncultured Mycobacteriaceae bacterium | 2D | 99.71 | 0 | GQ153275 | Mycobacterium colombiense |
1E | 97.36 | 0 | AY859686 | Mycobacterium moriokaense | 2E | 99.71 | 0 | GQ153275 | Mycobacterium colombiense |
1F | 98.91 | 0 | AY859686 | Mycobacterium moriokaense | 2F | 99.13 | 0 | EU274642 | Mycobacterium riyadhense |
1G | 98.52 | 0 | FJ605266 | Mycobacterium sp. DCY42 | 2G | 99.09 | 0 | EU274642 | Mycobacterium riyadhense |
1H | 98.31 | 0 | AY162028 | Mycobacterium sp. JS623 | 2H | 99.71 | 0 | GQ153275 | Mycobacterium colombiense |
1I | 97.53 | 4×10−145 | FJ538898 | Mycobacterium sp. GR-2001-270 | 2I | 98.85 | 0 | U06638 | Mycobacterium haemophilum |