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. 2013 Jul 18;8(7):e69363. doi: 10.1371/journal.pone.0069363

Figure 6. N-terminal residues of APP family ICDs are crucial for nuclear signaling capability.

Figure 6

(A) Confocal fluorescence images of HEK cells transfected with HA-Fe65, CFP-Tip60 and cotransfected with APP-Cit or the indicated APP-Cit mutation constructs. Scale bar represents 13 µm. (B) Co-immunoprecipitation of SBP-tagged APP or APP(VML646LLR) together with HA-tagged Fe65 using Dynabeads. (C) Western blot analysis of HEK cells transfected with APP-Cit, APLP1-Cit or APP(VML646LLR)-Cit constructs after 24 hour treatment with the γ-secretase inhibitor DAPT. AICD-Cit transfected cell lysate was loaded to identify ICD bands and the membrane was probed with anti-GFP antibody.