Table 1. Transcription ratio of the S. maltophilia R551-3 wild-type compared to the rpfF mutant strain for selected genes that code for products of important physiological role with regard to bacteria-plant interactions.
Physiological role | Gene/locus | Product/function | Fold change wild-type/rpfF mutant |
cell motility | Smal_1869 | Flagellar export protein FliQ | 35.1 |
fliN | flagellar motor switch protein FliN | 28.2 | |
Smal_1868 | flagellar biosynthetic protein | 17.9 | |
Smal_1877 | flagellar related ATPase | 17.4 | |
Smal_1881 | flagellar hook-basal body complex subunit | 12.9 | |
motC | flagellar motor protein | 9.9 | |
flgA | flagellar basal body P-ring biosynthesis protein | 4.5 | |
Smal_1874 | flagellar basal body-associated protein | 4.5 | |
Smal_1863 | cobyrinic acid ac-diamide synthase | 3.2 | |
Smal_1894 | flagellin | 3.1 | |
flgK | flagellar hook-associated protein | 3.1 | |
Smal_1909 | putative anti-sigma-28 factor | 2.4 | |
Smal_1876 | flagellar export protein | 2.3 | |
flgJ | flagellar rod assembly protein | 2.1 | |
chemotaxis | Smal_1907 | response regulator receiver CheW | 15.0 |
Smal_1846 | methyl-accepting chemotaxis sensor molecule | 6.3 | |
Smal_1855 | chemotaxis signal transducer | 2.7 | |
Smal_1845 | CheR-type chemotaxis methyltransferase | 2.7 | |
direct plant growth promoting | Smal_2304 | putative spermidine export protein * | 43.8 |
Smal_2305 | putative spermidine export protein * | 5.4 | |
Smal_3769 | spermidine synthase | 1.5 | |
Smal_1341 | spermidine/putrescine-binding periplasmic protein | 1.5 | |
LPS biosynthesis | Smal_0537 | permease | 2.1 |
Smal_1400 | 3-deoxy-manno-octulosonate cytidylyltransferase | 1.9 | |
Smal_3508 | UDP-N-acetylglucosamine pyrophosphorylase | 1.4 | |
biofilm formation | Smal_2718 | polysaccharide polymerase involved in biofilm formation | 3.8 |
Smal_0846 | serine-threonine phosphatase | 2.0 | |
Smal_2717 | polysaccharide deacetylase | -5.5 | |
iron transport | Smal_1803 | iron transport protein | 1.8 |
antibiotic resistance and multidrug pumps | Smal_2304 | multidrug resistance protein | 43.8 |
Smal_2305 | small multidrug resistance protein | 5.4 | |
Smal_2146 | beta-lactamase | 2.3 | |
Smal_1568 | multidrug efflux transporter (SmeW) | 2.7 | |
chaperone synthesis | Smal_1891 | flagellin-specific chaperone | 50.2 |
Smal_1916 | DnaJ class molecular chaperone | 15.0 | |
Smal_1271 | pili assembly chaperone | 14.2 | |
Smal_1576 | severe stress chaperone | 0.2 |
Genes listed here play crucial roles in plant colonization, biofilm formation, ecological persistence, biocontrol and plant growth promotion. *: Originally annotated as multidrug resistance protein, but blastp analysis revealed significant homology to the mdtJ, the spermidine export protein of the plant growth promoting and biocontrol agent S. r,hizophila DSM14405T.