Figure 4. Both, curcumin and AG490, rescue expression of p53 and pRb through suppression of HPV16 E6 and E7 expression.

(A–B) Effect of curcumin and AG490 on p53, pRb expression in cervical cancer cells. SiHa cells (1×10⁶ cells) treated with the curcumin (50 µM) or AG490 (100 μM) for indicated durations were tested for p53 and pRb expression by western blotting as described. (C) Curcumin and AG490-mediated abrogation of HPV16 E6 and E7 expression. SiHa cells (1×10⁶ cells) treated with curcumin (50 µM) or AG490 (100 μM) for 24 h were tested for HPV16 E6 and E7 expression by immunoblotting as described. Blots were stripped and re-probed with β-actin as loading control.