Table 1. Gating properties of DmNav variants with or without TipE or TEH1.
| Activation |
Fast Inactivation |
Slow inactivation |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| V1/2 (mV) |
k (mV) |
V1/2 (mV) |
k (mV) |
V1/2 (mV) |
k (mV) |
||||
| DmNav9-1 | -29.0 ± 1.1 | 5.5 ± 0.7 | -41.4 ± 0.8 | 4.8 ± 0.2 | -44.5 ± 1.8 | 5.6 ± 0.8 | |||
| DmNav9-1 + TipE | -26.2 ± 0.5 | 5.2 ± 0.2 | -42.1 ± 0.7 | 5.1 ± 0.1 | -44.9 ± 1.4 | 5.5 ± 0.2 | |||
| DmNav9-1 + TEH1 | -41.1 ± 1.1* | 4.6 ± 0.2 | -51.7 ± 0.6* | 5.0 ± 0.1 | -60.2 ± 0.5* | 5.0 ± 0.3 | |||
| DmNav26 | -25.7 ± 0.2 | 3.4 ± 0.3 | -34.1 ± 0.1 | 4.1 ± 0.2 | -45.6 ± 0.2 | 5.0 ± 0.3 | |||
| DmNav26 + TipE | -24.7 ± 0.6 | 3.4 ± 0.4 | -40.8 ± 0.4* | 4.2 ± 0.2 | -44.1 ± 0.7 | 4.5 ± 0.4 | |||
| DmNav26 + TEH1 | -33.6 ± 0.6* | 3.1 ± 0.4 | -42.6 ± 0.6* | 4.6 ± 0.1 | -50.6 ± 0.3* | 4.5 ± 0.5 | |||
| DmNav22 | -26.7 ± 1.1 | 5.8 ± 0.5 | -40.7 ± 0.6 | 4.8 ± 0.1 | -49.2 ± 0.2 | 3.7 ± 0.2 | |||
| DmNav22 + TipE | -25.1 ± 1.2 | 7.2 ± 0.5 | -38.9 ± 0.5 | 4.8 ± 0.2 | -45.5 ± 0.8 | 5.3 ± 0.2 | |||
| DmNav22 + TEH1 | -32.1 ± 0.7* | 6.2 ± 0.3 | -48.3 ± 0.6* | 5.2 ± 0.1 | -57.9 ± 0.7* | 4.8 ± 0.1 | |||
Data represent mean ± SEM for 12-20 oocytes. DmNav 9 1, DmNa v 26, and DmNav 22 are treated as control of each group.
*
Significantly different from that of DmNav channel only using one-way ANOVA with Scheffe’s post hoc analysis (p<0.05).