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. 2013 Jul 18;8(7):e68767. doi: 10.1371/journal.pone.0068767

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© 2013 Kim et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Measurement of GFP reporter activity, using Victor 3 and Opera reader, in CEMx 174-LTR-GFP CG8 cells upon HIV-1_LAI infection. B. Infection of CEMx 174-LTR-GFP CG8 and HeLa-LTR-GFP cells: Opera-acquired images showed infected (Panel b and d), unlike uninfected (Panel a and c) cells, carried the GFP reporter gene. Syncytia are indicated by red circles. C. Assay validation upon antiretroviral treatment: reference drugs were tested to evaluate the assay performance using an Opera reader. CEMx 174-LTR-GFP CG8 cells infected by HIV-1_LAI were treated with AZT (Panel b), nevirapine (Panel c) and saquinavir (Panel d). Images show nucleus detection in red and infected cells in green (Panel a: DMSO).