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. 2013 Jul 18;8(7):e68943. doi: 10.1371/journal.pone.0068943

Figure 2. Silica induced the expression of Egr-1 at levels of nuclear protein and mRNA.

Figure 2

(a) The dynamic expression of Egr-1 nuclear protein in A549 cells, which were exposed to silica for indicated times, was determined by western-blot using anti-Egr-1 antibody. Obvious increase of Egr-1 occurred from 30 minutes of exposure, and peaked after exposure for 60 minutes followed by gradually decrease and return to baseline by 480-minute treatment (2B). Silica induced the transcription of Egr-1 and mRNA level was determined by RT-PCR. Significant differences in Egr-1 protein and mRNA expression are noted at P<0.01(**).