Figure 6. Muscle regeneration is incomplete 30 d following denervation-devascularization (DD) injury in both young and aged mice. Representative images of transverse sections of EDL muscles from young (3 mo) and aged (22 mo) mice, 30 d (D30) following DD injury stained with H&E (A, A’, C, C’) and Gomori’s Trichrome (blue = collagen) (B, B’, D, D’). Images A’-D’ are higher magnifications of the boxed regions in A-D, respectively. Scale Bars: A-D = 200 μm; A’, C’ = 50 μm; B’, D’ = 100 μm. (E) Percentage of myofibers with central nuclei. (F) EDL muscle weight as percentage of body weight. (G) total number of myofibers. (H) Cross-sectional area of the EDL muscle. (I) Percentage of muscle area occupied by collagen. Statistical differences are indicated as follows: * p < 0.05, ** p < 0.01 compared with untreated age-matched group; ## p < 0.01 compared with young muscle of the same treatment group.