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. Author manuscript; available in PMC: 2013 Jul 18.
Published in final edited form as: Chembiochem. 2012 Nov 20;13(18):2722–2728. doi: 10.1002/cbic.201200525

Figure 1.

Figure 1

Self-assembly of organized immunofluorescent reporting complexes using modular dynamic DNA complexes. Target proteins are outfitted with a ssDNA tag using a primary antibody (Ab) and a DNA-conjugated secondary antibody. Strand displacement reactions are then performed to either (i) label the ssDNA tag directly with a linear, DNA probe complex that, in the present study, carries between 1 and 3 dye molecules, or to (ii and iii) build branched DNA complexes to increase the number of target sites for the linear probe’s displacement reactions. For simplicity, all reactions are shown using a linear probe possessing a single dye molecule and a quencher.