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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Clin Cancer Res. 2013 May 20;19(14):3844–3855. doi: 10.1158/1078-0432.CCR-13-0505

Fig 4. MHC class I blockade in AML targets triggers NK cell activation, effect enhanced with CD16x33 BiKE activation.

Fig 4

AML target cells were pre-incubated with 20 ug/mL of a pan-HLA blocking antibody (HP-1F7) for 30 mins prior to culturing with NK cells. Purified NK cells isolated from healthy donors (n=5) were cultured with de-novo CD33+ AML cells (E:T ratio of 2:1). Cells were treated with or without 5 ug/mL of CD16x33 BiKE as indicated and cultured for 4 hours. Aggregate data of NK cell intracellular CD107a degranulation, TNF-α, and IFN-γ for the indicated conditions were determined by flow cytometry. (n=5, *P<0.05; **P<0.01, ***P<0.001).