Fig. 3. Alteration of TBP expression affects RNA pol III-dependent transcription.
(A) overexpressing TBP enhanced Pol III gene transcription. HepG2-ADH cells were transiently transfected with a TBP expression plasmids or control vector for 48 hours. RT-qPCR was performed on RNA derived from these cells to measure pre-tRNALeu transcripts (B) Repression of TBP reduced RNA pol III-dependent transcription. HepG2-ADH cells were transfected with TBP siRNA or mismatch RNA (mm RNA) for 48 hours. RT-qPCR was performed on RNA isolated from these cells to measure pre-tRNALeu transcripts. The fold change was calculated by normalizing to the amount of transcript in cells transfected with vector control plasmid or mm RNA. The bars represent Mean ± SE of at least three independent determinations.