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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: Gene. 2013 Feb 20;526(1):54–60. doi: 10.1016/j.gene.2013.02.004

Fig. 4. Alcohol induces TBP promoter activity in HepG2-ADH cells, but not hepG2-vector cells.

Fig. 4

HepG2-ADH and HepG2-vector cells were transfected with TBP-Luc reporter construct for 48 h; the cells were starved in DMEM for 3 h. Cells were then treated with or without ethanol for another 1h. The cell lysates was extracted from these cells and luciferase activity assay was performed to measure the TBP-Luc activity as described in Materials and Methods. The change in TBP promoter activity was calculated relative to that with no ethanol treatment. The values represent mean ± SE from three independent experiments.