Figure 2. MiR-9, miR-124, and let-7d are significantly increased in SH-SY5Y cells after exposure to 10% conditioned media from HIV-infected MDM.
Terminally differentiated SH-SY5Y cells were exposed to high p24 (1,700 pg/mL) (black bars) and low p24 (900 pg/mL) (grey bars) conditioned media from HIV-1 infected MDM, and RNA isolated and quantified by Taqman qPCR for miR-9, miR-124, and let-7d; with U6 and RNU44 as endogenous controls and calibrated to the average time 0 values. The Log (RQ) values are plotted. *(P < 0.05 compared to time 0), error bars indicate standard deviation of four independent samples.