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. Author manuscript; available in PMC: 2014 May 7.
Published in final edited form as: Oncogene. 2012 Nov 26;32(45):5283–5291. doi: 10.1038/onc.2012.543

Figure 1. STK38 kinase mediates anti-IgM induced MYC down regulation and cell apoptosis in ST486 cell line.

Figure 1

STK38-WT and STK38-KD expression in the ST486 cell lines was induced by doxycycline treatment. Cross-linking anti-IgM antibody was performed 24 hours after doxycycline induction. Cells were cultured in the presence of anti-IgM for an additional 6 (A) or 24 hours B; (A) Apoptosis levels measured by flow cytometry using Annexin V binding assay. Bars represent percentage of Annexin V positive cells ±SEM. P-values are estimated by paired t-test analysis. (B) Immunoblot analysis of the total cell extracts showing anti-phosphorylated MYC (p-MYC), anti-HA STK38 fusion proteins (HA), MYC and GAPDH. Results are representative of three independent experiments.