Table 1.
Top 30 genes modulated in bronchial epithelial cells in response to exposure to Alternaria alternata spores.
| Rank | Gene symbol | Fold change | Description | Genbank ID | Gene ontology biological process |
|---|---|---|---|---|---|
| 1 | MXl | 66.2 | Myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 (mouse) | NM_002462 | Induction of apoptosis, defense response, signal transduction, response to virus, cytokine-mediated signaling pathway |
| 2 | IFI44L | 64.1 | Interferon-induced protein 44-like | NM_006820 | Immune response |
| 3 | IFI44 | 39.2 | Interferon-induced protein 44 | BE049439 | Response to virus |
| 4 | XAFl | 31.2 | XIAP associated factor 1 | AI859280 | Apoptosis, cytokine-mediated signaling pathway, type 1 interferon-mediated signaling pathway |
| 5 | OAS2 | 30.8 | 2′-5′-oligoadenylate synthetase 2, 69/71 kDa | AI651594 | Immune response, cytokine-mediated signaling pathway, interferon-gamma-mediated signaling pathway |
| 6 | IFI27 | 25.2 | Interferon, alpha-inducible protein 27 | NM_005532 | Induction of apoptosis by extracellular signals, activation of pro-apoptotic gene products, cytokine-mediated signaling pathway, type 1 interferon-mediated signaling pathway |
| 7 | IFIH1 | 19.3 | Interferon induced with helicase C domain 1 | AL080107 | Negative regulation of type 1 interferon production, positive regulation of interferon-alpha production, positive regulation of interferon-beta production |
| 8 | CMPK2 | 18.7 | Cytidine monophosphate (UMP-CMP) kinase 2, mitochondrial | AI742057 | Pyrimidine nucleotide biosynthetic process, dTDP biosynthetic process, cellular response to lipopolysaccharide |
| 9 | MX2 | 17.9 | Myxovirus (influenza virus) resistance 2 | NM_002463 | Defense response, cytokine-mediated signaling pathway, type 1 interferon-mediated signaling pathway |
| 10 | IFIT1 | 16.4 | Interferon-induced protein with tetratricopeptide repeats 1 | NM_001548 | Intracellular transport of viral proteins in host cell, cytokme-mediated signaling pathway, negative regulation of protein binding, positive regulation of viral genome replication |
| 11 | HERC6 | 15 | Hect domain and RLD 6 | NM_017912 | Protein modification process, protein ubiquitination involved in ubiquitin-dependent protein catabolic process |
| 12 | HERC5 | 13.9 | Hect domain and RLD 5 | NM_016323 | Regulation of cyclin-dependent protein kinase activity, protein modification process, response to virus, ISGl5-protein conjugation, negative regulation of type 1 interferon production |
| 13 | IFIT3 | 13.7 | Interferon-induced protein with tetratricopeptide repeats 3 | NM_001549 | Cytokine-mediated signaling pathway, cellular response to interferon-alpha, type 1 interferon-mediated signaling pathway |
| 14 | IFIT2 | 13.6 | Interferon-induced protein with tetratricopeptide repeats 2 | BE888744 | Response to virus, cytokine-mediated signaling pathway, negative regulation of protein binding, cellular response to interferon-alpha |
| 15 | CDC2O | 13.5 | Cell division cycle 20 homolog (S. cerevisiae) | NM_001255 | Cell cycle checkpoint, M phase of mitotic cell cycle, mitotic prometaphase |
| 16 | OAS1 | 13.2 | 2′,5′-oligoadenylate synthetase 1, 40/46kDa | NM_002534 | Immune response, cytokine-mediated signaling pathway, interferon-gamma-mediated signaling pathway, type 1 interferon-mediated signaling pathway |
| 17 | TNFSF10 | 13.2 | Tumor necrosis factor (ligand) superfamily, member 10 | U57O59 | Apoptosis, induction of apoptosis, activation of caspase activity, immune response |
| 18 | DDX58 | 12.1 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 | AI304317 | Defense response to virus by host, detection of virus, negative regulation of type 1 interferon production, positive regulation of interferon-beta production |
| 19 | RARRES1 | 12.1 | Retinoic acid receptor responder (tazarotene induced) 1 | NM_002888 | Negative regulation of cell proliferation |
| 20 | ISG15 | 12 | ISG15 ubiquitin-like modifier | NM_005101 | Cell–cell signaling, response to virus, cytokine-mediated signaling pathway, modification-dependent protein catabolic process, ISGl5-protein conjugation |
| 21 | USP18 | 11.9 | Ubiquitin specific peptidase 18 | NM_017414 | Cytokine-mediated signaling pathway, type 1 interferon-mediated signaling pathway, regulation of type 1 interferon-mediated signaling pathway |
| 22 | RTP4 | 10 | Receptor (chemosensory) transporter protein 4 | NM_022147 | Protein targeting to membrane |
| 23 | STAT1 | 9.8 | Signal transducer and activator of transcription 1, 9lkDa | NM_007315 | Regulation of transcription, transcription from RNA polymerase II promoter, induction of apoptosis, activation of caspase activity, signal transduction, JAK-STAT cascade, tyrosine phosphorylation of STAT protein |
| 24 | OASL | 8.5 | 2′-5′-oligoadenylate synthetase-like | NM_003733 | Immune response, interferon-gamma-mediated signaling pathway, type 1 interferon-mediated signaling pathway |
| 25 | DDX6O | 8 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 | NM_017631 | – |
| 26 | IFITM1 | 7.8 | Interferon induced transmembrane protein 1 (9-27) | NM_003641 | Cell surface receptor linked signaling pathway, negative regulation of cell proliferation, response to virus, cytokine-mediated signaling pathway |
| 27 | OAS3 | 7.7 | 2′-5′-oligoadenylate synthetase 3, lOOkDa | R13458 | Immune response, cytokine-mediated signaling pathway, interferon-gamma-mediated signaling pathway, type 1 interferon-mediated signaling pathway |
| 28 | DHRS3 | 7.3 | Dehydrogenase/reductase (SDR family) member 3 | NM_004753 | Retinol metabolic process |
| 29 | PARP9 | 7.2 | Poly (ADP-ribose) polymerase family, member 9 | AI738416 | Cell migration |
| 30 | IFI6 | 7 | Interferon, alpha-inducible protein 6 | NM_022873 | Immune response, cytokine-mediated signaling pathway, negative regulation of caspase activity |
The top 30 differentially expressed (up-regulated) genes are shown along with the gene description, the relative fold change of the target genes, and the NCBI GenBank ID. A description of Gene Ontology (GO) annotation of the biological processes is also depicted.