Table 1. Bacterial strains and plasmids.

S.N.	Strain/Plasmid/Primer Name	Genotype/Phenotype/Sequence Description	Reference/Source/Remark
	Strains
1	E. coli DH5α	fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17	58
2	E coli BL21 DE3	hsdS gal(λcIts857 ind1 sam7 nin5lac UV5 T7 gene 1	59
3	E. coli pir116	F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG pir-116(DHFRv)	EPICENTRE Biotechnologies
4	Acinetobacter sp. DS002	Smr and Cmr	This work
	Plasmids
5	pET23b	Apr, Expression vector	Novogen
6	pTRW1	Apr, repA cloned in pET23b from predicted start codon TTG	This work
7	pTRW2	Apr, repA cloned in pET23b from its first ATG codon	This work
8	pTRM	Expression plasmid coding for RepA Y265F	This work
9	pTS236K	Rescued cloned pTS236 having minitransposon in the intergenic region of repA and orf96	This work
10	pTS236K1	pTS236 having kanamycin cassette at unique Mlu I site found in the intergenic region of repA and orf106	This work
11	pT106M	pTS236-K having termination codon immediately downstream to the start codon of orf106	This work
12	pT96M	pTS236-K having termination codon immediately downstream to the start codon of orf96	This work