Figure 1.

Time points for imaging during bubble circulation and interframe filtered volumetric imaging. A: Broadband (T1R5.5) RF data (yellow bar) were acquired at 2 minutes (when both circulating microbubbles and bound microbubbles were present in the blood stream) and 7 minutes (primarily bound microbubbles) after targeted microbubble injection. Multi-pulse (CPS) image data were acquired 7 minutes after the injection of microbubbles (red bar); thus, most circulating microbubbles had cleared from the vasculature while bound microbubbles were retained. B: Multiple image frames were acquired in each plane and then algebraically combined and filtered to differentiate selectively image bound microbubbles. The ultrasound probe was then scanned in the third dimension to capture a volumetric data set.