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. 2013 Mar 12;22(15):2211–2220. doi: 10.1089/scd.2012.0611

FIG. 1.

FIG. 1.

Hoxa9 and Hoxa10 are expressed in early cardiac progenitor cells of the cardiac crescent. (A) Individual WT and Nkx2-5 null cardiac crescent stage embryos were bisected and EYFP-positive cells were collected from the right and left side of the crescent. Left WT mean=2044, SD=499, n=5; left null mean=1967, SD=200, n=4; right WT mean=2263, SD=294, n=5; right null mean=2167, SD=252, n=4. (B) Transcriptome analysis of the WT and Nkx2-5 null left versus right side of the crescent. Note a significant induction of cardiac structural transcripts in the left versus right WT crescent. This left-sided enrichment is absent in the Nkx2-5 null crescent. The right-sided enrichment in expression is largely unaltered in the absence of Nkx2-5. Significant differential transcript expression is denoted by a green highlight (upregulated) or a red highlight (downregulated). (C) quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) of selected trannscripts in WT (black bar) and null (open bar) right and left crescent.