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. 2013 Mar 12;22(15):2211–2220. doi: 10.1089/scd.2012.0611

FIG. 2.

FIG. 2.

Characterization of cardiac Hoxa10 expression. (A) Analyses of enrichment of Hoxa9 and Hoxa10 in the EYFP-positive Nkx2-5 expressing cells versus EYFP-negative cells collected from the cardiac crescent using qRT-PCR; *P<0.05, n=3. (B) qRT-PCR reveals Hoxa9 and Hoxa10 are enriched in the E8.25 (5–7 somite) heart compared to the remaining segments of the embryo; *P<0.05, n=3. (C) Cardiac Hoxa9 and Hoxa10 expression is rapidly downregulated during cardiac development as assessed by qRT-PCR; *P<0.05, n=3. (D) Analyses of fold change in the expression of the Hoxa9, Hoxa10, and Nkx2-5 from GFP-positive Nkx2-5 expressing cells versus GFP negative obtained from embryoid bodies (EBs) harvested at day 4 using qRT-PCR; *P<0.05, n=3. (E) Western analysis of EB day 4 GFP-positive Nkx2-5 expressing cells confirm Hoxa10 protein expression.