FIG. 4.

CD133⁺CD140a⁻ antigenically defines multipotential neural stem/progenitor cells. (A–D) The lineage potential of each fraction was determined by plating spheres into prodifferentiation conditions and immunostaining for markers of neuron (ßIII-tubulin/TuJ1, green), astrocyte (GFAP, blue), and oligodendrocyte cells (O4, red). Scale bar=200 μm. (E) The number of spheres containing all three lineages (N/A/O), neurons/astrocytes (N/A), only glial lineages (A/O) or only astrocytes (A) were quantified (mean±SEM, n=6 fetal samples). *, **, and *** indicate Dunnett's post hoc P<0.05, P<0.01, and P<0.001, respectively, compared with CD133⁻CD140a⁻. Color images available online at www.liebertpub.com/scd