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. 2013 Jun 18;32(14):2073–2085. doi: 10.1038/emboj.2013.145

Figure 2.

Figure 2

MSI1 is needed for trimethylation of H3K27. (A) Global H3K27me3 levels are reduced in msi1–cs plants. Total protein levels were analysed by quantitative immunoblotting using anti-H3K27me3 and anti-H3 antibodies in Col and msi1–cs plants. Shown are mean ±s.d. (n=3). (B) H3K27me3 is reduced at the chromatin of EMF target genes in msi1–cs plants. Top: genomic structure of SEP3, MAF5, AG, AT3G28007 and ACTIN7. Black lines, introns; wide bars, exons. Arrows represent the position of primers used for qPCR. Values are recovery as percent of input; shown are mean ±s.d. (n=3).