Figure 1. ESE-1 induction in response to Ang II in vitro and in vivo. (a) ESE-1 expression as measured by PCR in HASMCs after Ang II stimulation (100nmol/l). (b) ESE-1 expression in HASMCs under Ang II stimulation by real-time Q-PCR. (c) ESE-1 expression in thoracic aorta under Ang II infusion (1.4mg/kg/day) for 3, 7, and 14 days by PCR. (d) Immunohistochemical analysis of ESE-1 expression after infusion of Ang II for 2 weeks in C57BL/6 compared to vehicle control. Original magnification ×250. HASMC, human aortic smooth muscle cell. Ang II, angiotensin II; ESE-1, epithelium-specific ETS transcription factor-1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PCR, polymerase chain reaction.