Skip to main content
. 2013 May 2;4(7):1071–1080. doi: 10.1021/cn4000346

Figure 2.

Figure 2

SR-FTIR-FPA imaging of a Purkinje neuron within the cerebellum. (A) Optical image of the unstained tissue section with location of neuron soma drawn free hand in black, the nucleus shown with the white arrowhead. Black square indicates region analyzed with SR-FTIR-FPA imaging. (B) Optical image of cresyl biolet stained section analyzed with SR-FTIR-FPA imaging. Cresyl violet stain performed after spectroscopic analysis. (C) False-color functional group image generated from area underneath the lipid ν(C=O) band (1750–1710 cm–1), to reveal the presence of neuron soma. (D) False-color functional group image generated from the ratio of the area underneath the νs(CH3) band (2880–2865 cm–1) and νs(CH2) band (2860–2845 cm–1), to reveal the presence of neuron nuclei. Scale bars = 10 μm.