Figure 7.

Determination of relative lactate concentration from FTIR spectra. (A) A representative example of the curve fitting approach used to determine the integrated area underneath the band centered at 1127 cm^–1. This approach was used to demonstrate the significant increase in integrated band area for the band centered at 1127 cm^–1 from the average spectra collected from Purkinje neurons relative to the surrounding molecular and granular layer tissue presented in Table 1. (B) Representative second-derivative spectra from a Purkinje neuron and surrounding molecular and granular layer tissue, highlighting the increase intensity at 1127 cm^–1 from spectra collected within the Purkinje neuron.