Figure 6.

Increase in NR2B protein following D1DR stimulation and cocaine self-administration does not correspond to an increased contribution of NR2B to whole-cell NMDAR currents. A, Quantitative Western blot analyses of NMDAR subunit protein levels. Protein levels were examined in nucleus accumbens shell homogenates from five animals in each group (*p < 0.05 vs nontreated saline, two-way ANOVA). B, Effects of NR2B antagonist, Ro 25-6981 (0.05, 0.5, 5, 50 μm), on currents evoked by whole-cell application of NMDA (100 μm). For each neuron, the responses were normalized to currents evoked by NMDA in the absence of Ro 25-6981. Notice large variability in responses. There were no significant differences between groups (n = 4–9 cells at each concentration).